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Analyzing Oxygen Consumption Rate in Primary Cultured Mouse Neonatal Cardiomyocytes Using an Extracellular Flux Analyzer
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Analyzing Oxygen Consumption Rate in Primary Cultured Mouse Neonatal Cardiomyocytes Using an Extracellular Flux Analyzer

Analyzing Oxygen Consumption Rate in Primary Cultured Mouse Neonatal Cardiomyocytes Using an Extracellular Flux Analyzer

DOI:
10.3791/59052-v

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11:26 min

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February 13, 2019

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, , , , , , , , , , ,
1Division of Cardiology, Department of Medicine,University of California San Diego, 2Department of Pharmacology,University of California San Diego, 3Cardiology Section, Department of Medicine,Veterans Administration Healthcare, San Diego

Chapters

  • 00:04Title
  • 01:13Harvesting and Pre-digestion of Hearts from Neonatal Mice
  • 02:43Enzymatic Digestion and Cell Plating
  • 06:56Oxygen Consumption Assay Using a 96-well Format Extracellular Flux Analyzer
  • 09:03Results: Mitochondrial Stress Assay
  • 10:34Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to illustrate how to use mouse neonatal cardiomyocytes as a model system to examine how various factors can alter oxygen consumption in the heart.

Tags

Keywords: Neonatal CardiomyocytesOxygen Consumption RateExtracellular Flux AnalyzerMitochondrial FunctionMetabolic ParametersCell CultureTrypsinCollagenase

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