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Wholemount hibridación In Situ de embriones de Astyanax
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Wholemount In Situ Hybridization for Astyanax Embryos
DOI:

09:56 min

March 02, 2019

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Chapters

  • 00:04Title
  • 00:40Day 1: Rehydration
  • 01:30Day 1: Digestion and Fixation
  • 02:42Day 1: Prehybridization and Day 2: Hybridization
  • 03:46Day 3: Solution Preparation and Probe Removal
  • 05:02Day 3: Blocking
  • 05:42Day 4: MABT Rinses
  • 06:34Day 5: Probe Visualization
  • 08:00Results: Analysis of Mounted Astyanax Embryos
  • 09:37Conclusion

Summary

Automatic Translation

Este protocolo permite la visualización de la expresión génica en embrionario Astyanax cavefish. Este enfoque ha sido desarrollado con el objetivo de maximizar la señal de la expresión del gene, minimizando tinción de fondo inespecífica.

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