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三维水凝胶中全球细胞基质金属蛋白酶和代谢活性的测定

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels

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三维水凝胶中全球细胞基质金属蛋白酶和代谢活性的测定

DOI:

10.3791/59123-v

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07:39 min

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January 22, 2019

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Abdulaziz S. Fakhouri^2,3, Jennifer L. Leight²

¹Department of Biomedical Engineering,The Ohio State University, ²The Ohio State University Comprehensive Cancer Center,Arthur G. James Cancer Hospital and Richard J. Solove Research Institute, ³Biomedical Technology Department,King Saud University

Chapters

00:04Title
00:46Assay Media and Hydrogel Preparation
01:41Encapsulate Cells in Hydrogels
03:51MMP and Metabolic Activity Measurement
05:08Results: Measuring MMP and Metabolic Activities of Cells Cultured in 3D Fluorogenic Hydrogels
06:46Conclusion

Summary

Automatic Translation

本文提出了一种用荧光基质金属蛋白酶 (mmp) 可降解肽对聚 (乙二醇) (peg) 水凝胶进行包封和培养的方案。细胞 mmp 和代谢活性直接从水凝胶培养物测量使用标准的微板读取器。

Tags

Keywords: 3D Hydrogels Cellular Function Fluorescent Sensor Protease Activity High-throughput Drug Screening Hydrogel Preparation Cell Encapsulation A375 Melanoma Cells PBS Buffer 96-well Plate

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