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Immunology and Infection

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一种评估和定量中性粒细胞外细胞陷阱形成的高通量检测

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation

Please note that all translations are automatically generated. Click here for the English version.

一种评估和定量中性粒细胞外细胞陷阱形成的高通量检测

DOI:

10.3791/59150-v

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09:59 min

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January 29, 2019

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Eline J. Arends*¹, Laura S. van Dam*¹, Tineke Kraaij, Sylvia W.A. Kamerling, Ton J. Rabelink, Cees van Kooten, Y.K. Onno Teng

¹Department of Nephrology,Leiden University Medical Center

Chapters

00:04Title
01:13Isolation of Healthy Neutrophils
03:42Red Fluorescent Cell Labelling of Neutrophils
05:10Induction of Neutrophil Extracellular Trap Formation
06:42NET Visualization with 3D High-content, High-resolution Immunofluorescence Confocal Microscopy and Analysis of NET Formation
07:59Results: Assessment and Quantification of Neutrophil Extracellular Trap Formation
09:26Conclusion

Summary

Automatic Translation

该协议描述了一种高度敏感和高吞吐量的中性粒细胞细胞外陷阱 (net) 检测方法, 用于通过免疫荧光三维共聚焦显微镜对体外网络形成进行半自动定量。该方案可用于评估 net 在不同刺激后的形成和降解, 并可用于研究潜在的 net 靶向疗法。

Tags

Keywords: Neutrophil Extracellular Traps (NETs)High-throughput Assay Immunofluorescence Confocal Microscopy Autoimmune Diseases ANCA-associated Vasculitis Systemic Lupus Erythematosus Neutrophil Isolation Density Gradient Centrifugation

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