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Siembra e implantación de un injerto traqueal tejido-dirigida biosintético en un modelo murino
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1Department of Otolaryngology Head & Neck Surgery,Nationwide Children’s Hospital, 2The Ohio State University College of Medicine, 3Center for Regenerative Medicine,Research Institute at Nationwide Children’s Hospital, 4Center for Perinatal Research,Nationwide Children’s Hospital, 5Nanofiber Solutions, Inc., 6Department of Pediatric Surgery,Nationwide Children’s Hospital
Chapters
- 00:04Title
- 01:15Scaffold Manufacturing
- 02:50Bone Marrow-Derived Mononuclear Cell (BM-MNC) Harvest
- 04:40Cell Seeding
- 05:21Graft Implantation
- 08:28Results: Representative Basal Epithelial Cell and Stenotic Graft Region Analyses
- 09:27Conclusion
Estenosis del injerto constituye un obstáculo fundamental en ingeniería tisular de la vía aérea recambio. Para investigar los mecanismos celulares subyacentes estenosis, utilizamos un modelo murino de reemplazo traqueal ingeniería tisular con células mononucleares de médula ósea (BM-MNC). Aquí detallamos nuestro protocolo, incluyendo fabricación de andamio, BM-MNC aislamiento, siembra del injerto y la implantación.
