Journal
/
Bioengineering
/
趋化梯度多细胞响应的三维分析
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
3D Analysis of Multi-cellular Responses to Chemoattractant Gradients
Please note that all translations are automatically generated. Click here for the English version.

趋化梯度多细胞响应的三维分析

DOI:
10.3791/59226-v

•

05:57 min

•

May 24, 2019

•

, , , ,
1Department of Biomedical Engineering and Yale Systems Biology Institute,Yale University, 2Department of Biomedical Engineering,Johns Hopkins University, 3Center for Cell Dynamics and Department of Cell Biology,Johns Hopkins University

Chapters

  • 00:04Title
  • 00:34Mesofluidic Device Fabrication
  • 02:20Collagen Injection, 3D Imaging, and Quantification
  • 04:20Results: Representative Ligand Gradient Evaluation and Cellular Gradient Reponses
  • 05:23Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了一种方法来构建设备的三维培养和实验与细胞和多细胞有机体。该装置允许分析在具有明确趋化因子梯度的三维微环境中对可溶性信号的细胞反应。在检测弱噪声输入时, 有机体优于单个细胞。

Tags

Keywords: 3D AnalysisMulti-cellular ResponseChemoattractant GradientsPDMS DeviceMesofluidic DeviceStereo LithographyVacuum DegassingPDMS CuringCell LoadingCollagen Cell SuspensionCell Culture3D Physiological Environment

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59226?article_type=v

循环压力为生物反应器的设计体外主动脉心脏瓣膜的研究
check_url/59226?article_type=v

创建的胶粘剂和可溶性梯度荧光显微镜成像细胞迁移
check_url/59226?article_type=v

相关显微镜的动力相互作用的三维结构分析
check_url/59226?article_type=v

多尺度孔梯度，聚电解质和间接监测改性金属植入物<em>在体内</em
check_url/59226?article_type=v

一种意志的运动反应的及时性和准确性，以振动触觉刺激评价方法
check_url/59226?article_type=v

细胞迁移研究在垂直化学和氧梯度聚二甲基硅氧烷的聚碳酸酯微流体装置
check_url/59226?article_type=v

Bioink Precellularization 无源混合装置打印软骨及皮肤组织类似物的应用
check_url/59226?article_type=v

使用顺序 3D-3D 注册进行四维 CT 分析
check_url/59226?article_type=v

在微流体进化加速器上生成跨癌症人群的异构药物梯度，用于实时观察
check_url/59226?article_type=v

通过仪器和分析工具量化动眼神经反应和调节

Read Article