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1Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine,University of California, Los Angeles, 2Vatche and Tamar Manoukian Division of Digestive Diseases, Integrated Molecular Technologies (IMT) Core, Department of Medicine,University of California, Los Angeles, 3Division of Hematology and Oncology, Department of Medicine,University of California, Los Angeles
Chapters
- 00:04Title
- 00:40Vector Design and Generation
- 01:52Virus Generation
- 04:24Particle Count using p24 Enzyme-linked Immunosorbent Assay Kit
- 07:07dCas9 and gRNA Transduction, Selection, and Clone Creation
- 10:18Results: Using CRISPR Technology to Overexpress Multiple Splice Variants of a lncRNA
- 12:08Conclusion
Traditionelle cDNA-baserede overekspression teknikker har en begrænset anvendelighed for overekspression af lange noncoding RNA'er på grund af deres flere splice formularer med potentielle funktionalitet. Denne revision rapporterer en protokol, ved hjælp af CRISPR teknologi til overexpress flere splice varianter af en længe noncoding RNA.
