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工程神经前体宫内移植,用于Vivo神经结构研究
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Intraventricular Transplantation of Engineered Neuronal Precursors for In Vivo Neuroarchitecture Studies
Please note that all translations are automatically generated.
Click here for the English version.
工程神经前体宫内移植,用于Vivo神经结构研究
DOI:
10.3791/59242-v
•
15:00 min
•
May 11, 2019
•
Simone Chiola
,
Manuela Santo
,
Antonello Mallamaci
1
Laboratory of Cerebral Cortex Development
,
SISSA
Chapters
00:00
Title
00:42
The Procedure
01:41
Preparation of the Green Pool
02:09
Engineering the Green Sub-pools
03:19
Preparing Borosilicate Needles
04:17
Disinfecting the Hood and Preparing Cell Tracer
04:48
Assembling the Injection Tube
05:20
Mixing “Test” and “Control” Green Sub-Pools
06:24
Intraventricular Transplantation
09:11
Histology and Immunoflourescence
10:09
Morphometry
12:52
Results: Neuroarchitectural Analysis of Neurons Overexpressing a Neocortical Patterning Gene
14:17
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该方法基于神经元前体的体外慢病毒工程,将其共同移植到野生型大脑中,并配对对"测试"和"控制"衍生物的形态学评价,从而能够精确建模新皮质的体内基因控制神经元形态,以简单和负担得起的方式。
Tags
Keywords: Intraventricular Transplantation
Engineered Neuronal Precursors
In Vivo Neuroarchitecture Studies
Gene Expression Manipulation
Cortical Neuronal Architecture
Tau EGFP Transgenic Embryos
Lentiviral Infection
Neurosphere
Immunofluorescence
Morphometric Parameters
Precursor Pool
Tet-Off System
Doxycycline
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