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JoVE Journal Developmental Biology
Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation
 

Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation

Article DOI: 10.3791/59299-v • 08:47 min • March 15th, 2019
March 15th, 2019 •
1, 1
1Department of Biology, Wesleyan University

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This paper presents a surgical method for dissecting Drosophila pupal retinas along with protocols for the processing of tissue for immunohistochemistry, western analysis, and RNA-extraction. 

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Keywords: Drosophila Pupal Retina Immunohistochemistry Western Analysis RNA Isolation Dissection Eye-brain Complex Microdissection Forceps PBS Fixative
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