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Differentiation Capacity of Human Aortic Perivascular Adipose Progenitor Cells
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JoVE Journal Biology
Differentiation Capacity of Human Aortic Perivascular Adipose Progenitor Cells

Differentiation Capacity of Human Aortic Perivascular Adipose Progenitor Cells

DOI:
10.3791/59337-v

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10:43 min

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March 05, 2019

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, , , ,
1Maine Medical Center Research Institute

Chapters

  • 00:04Title
  • 00:40Culture Human PVAT Cells from the Stromal Vascular Fraction
  • 05:04Plate and Induce Adipogenic, Osteogenic, and Chondrogenic Lineages
  • 08:05Culture Adipogenic, Osteogenic, and Chondrogenic Lineages for 14 Days
  • 08:20Results: Progenitor Cells from Human Aortic Perivascular Adipose Tissue of Coronary Artery Bypass Grafting Patients Exhibit Adipogenic and Chondrogenic Differentiation but Fail to Commit to Ostegenic Lineages
  • 09:59Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to test the ability of progenitor cells derived from human perivascular adipose tissue to differentiate into multiple cell lineages. Differentiation was compared to mesenchymal stem cells derived from human bone marrow, which is known to differentiate into adipocyte, osteocyte, and chondrocyte lineages.

Tags

Adipose Progenitor CellsVascular MicroenvironmentCardiovascular DiseaseDissociationStromal Vascular FractionMultipotent Progenitors

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