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Chapters
- 00:04Title
- 01:00Sectioning of Human Skin Dermis into Papillary and Reticular Layers
- 02:38Separation and Enzymatic Digestion of Epidermis and Dermis
- 03:52Preparation of Single Cell Suspension and Erythrocyte Lysis
- 05:58Culturing of Fibroblasts and Adipogenesis Assay
- 07:04Results: FAP+CD90-, FAP+CD90+ and FAP-CD90+ Fibroblasts Differ in Dermal Localization, Gene Expression and Functionality
- 08:44Conclusion
Dette manuskript beskriver en FACS-baseret protokol til isolering af papillar og rekulære fibroblaster fra menneskehud. Det omgår in vitro-kultur, som var uundgåelig med den almindeligt anvendte isolation protokol via eksplantat kulturer. De udstammede fibroblast undersæt er funktionelt adskilte og udviser differentialgenekspression og lokalisering inden for dermis.
Tags
Keyword Extraction:Papillary FibroblastsReticular FibroblastsHuman SkinFluorescence-activated Cell SortingFACSDermal Fibroblast SubsetsSkin PathologiesCancerInflammatory Skin DiseasesFull-thickness DermisSubcutaneous Fat LayerPapillary DermisReticular DermisEnzymatic DigestionDissociating Enzyme Solution
