JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biochemistry
/
核糖体组蛋白的体外泛化和二次基质测定
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biochemistry
In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
Please note that all translations are automatically generated.
Click here for the English version.
核糖体组蛋白的体外泛化和二次基质测定
DOI:
10.3791/59385-v
•
11:36 min
•
July 25, 2019
•
Louis Masclef*
1
,
Uriarte Maxime*
1
,
Oumaima Ahmed
,
Nadine Sen Nkwe
,
Haithem Barbour
,
Nicholas V.G. Iannantuono
,
Amina Boubekeur
,
Salima Daou
2
,
El Bachir Affar
2
1
Maisonneuve-Rosemont Hospital Research Center and Department of Medicine
,
University of Montréal
,
2
Lunenfeld-Tanenbaum Research Institute
,
Sinai Health System
Chapters
00:04
Title
01:01
GSH-agarose Affinity Purification of the GST-RING1B(1-159)-BMI1 (1-109) E3 Ubiquitin Ligase Complex
02:16
Purification of the BAP1/DEUBAD (ASXL2) Deubiquitinase Complex
04:58
Purification of the Nucleosomes from HEK293T Cells
07:38
Nucleosome Ubiquitination Assay Using BMI1/RING1B E3 Ubiquitin Ligase Complex and In Vitro Nucleosomal H2A DUB Assay Using BAP1/DEUBAD
09:05
Results: Purification, Ubiquitination, and Deubiquitination
10:40
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
泛化是一种翻译后修饰,在细胞过程中起着重要作用,并且通过二足化进行紧密协调。两种反应的缺陷是人体疾病的基础。我们提供使用纯化成分在体外进行泛化和二次脱体反应的协议。
Tags
Keywords: In Vitro Ubiquitination
Deubiquitination
Nucleosomal Histones
Purified Components
Ubiquitination Mechanisms
Deubiquitination Mechanisms
Enzyme Mutations
Human Disease
High-throughput Screening
Small Molecule Inhibitors
Therapeutics
Bacteria Lysis
PMSF
Antiprotease Cocktail
GSH Beads
Chromatography Column
Glutathione
His-BAP1
MBP-DEUBAD
Nickel NTA Beads
Imidazole
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
check_url/59385?article_type=v
自动声胶肽受体激动剂的效价测定测定的连续稀释
check_url/59385?article_type=v
核小体的重建与差异同位素标记的姐妹组蛋白
check_url/59385?article_type=v
用天然电泳,凝胶电泳和电解法分析线粒体电子传递链的超复合物
check_url/59385?article_type=v
线虫
线虫中乳酸和丙酮酸细胞的小尺度比色测定
check_url/59385?article_type=v
低聚活性的体外定量
check_url/59385?article_type=v
生理活性类囊体的分离及其在能量依赖性蛋白转运检测中的应用
check_url/59385?article_type=v
通过肽浓缩和质谱检测蛋白质泛化位点检测
check_url/59385?article_type=v
富玛莱酸氢酶含含蛋白质的表达、纯化、结晶和酶测定
check_url/59385?article_type=v
RING-E3型无二体气体在Vitro和Planta中的功能特性
check_url/59385?article_type=v
基于无标签阻抗的 GPCR 检测中用于提高吞吐量的逐步加量协议
Read Article