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Excitation-scanning Hyperspectral Imaging mikroskopi til effektivt at diskriminere fluorescens signaler
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JoVE Journal Engineering
Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Excitation-scanning Hyperspectral Imaging mikroskopi til effektivt at diskriminere fluorescens signaler

DOI:
10.3791/59448-v

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00:07 min

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August 22, 2019

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, , ,
1Department of Chemical and Biomolecular Engineering,University of South Alabama, 2Center for Lung Biology,University of South Alabama, 3Department of Pharmacology,University of South Alabama

Chapters

  • 00:04Title
  • 00:44Excitation-Scanning Hyperspectral Imaging Microscopy
  • 04:12Image Analysis
  • 05:41Results: Excitation-Scanning Hyperspectral Imaging
  • 06:43Conclusion

Summary

Automatic Translation

Automatic Translation

Spectral Imaging er blevet en pålidelig løsning til identifikation og adskillelse af flere fluorescens signaler i en enkelt prøve og kan let skelne signaler af interesse fra baggrund eller autofluorescens. Excitation-scanning hyperspectral Imaging forbedrer denne teknik ved at reducere den nødvendige billed erhvervelse tid samtidig øge signal-til-støj-forholdet.

Tags

Keywords: Excitation-scanningHyperspectral ImagingFluorescence SignalsSpectral Imaging MicroscopySupercontinuum LaserSpinning DiskLaser Scanning Confocal MicroscopeFluorescent LabelsSample PreparationImage AcquisitionImage AnalysisImageJ

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