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En sammenfallende strategi for generering av en nesten sekvensielt cDNA bibliotek fra ubrukte Pacific østers
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
A Converging Strategy for the Generation of a Virtually Sequenced cDNA Library from Unreferenced Pacific Oysters
DOI:

12:14 min

June 13, 2019

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Chapters

  • 00:04Title
  • 01:10RNA Isolation by Guanidinium Thiocyanate-phenol Extraction and cDNA Library Generation by Reverse Transcription
  • 04:35Mitochondrial Gene Amplification, Purification, Sequencing and Comparison
  • 07:27Applying the cDNA Library for Cloning the Genes MgUGD and MgUXS
  • 08:42Expression and Activity Tests of MgUGD and MgUXS
  • 10:32Results: Sequencing and Mass Spectrometry Results
  • 11:33Conclusion

Summary

Automatic Translation

Vi beskriver en strategi for hvordan du bruker RNA prøver fra ubrukte Pacific østers prøver, og evaluere genetisk materiale ved sammenligning med offentlig tilgjengelige Genova data for å generere et tilnærmet sekvensielt cDNA bibliotek.

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