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En avansert murine modell for alkoholfrie steatohepatitis i samarbeid med type 2 diabetes
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1Department of Endocrinology & Metabolism Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin,Humboldt-Universität zu Berlin, 2Berlin Institute of Health (BIH), 3Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin,Humboldt-Universität zu Berlin, 4DZHK (German Centre for Cardiovascular Research), 5Institute of Medical Immunology, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin,Humboldt-Universität zu Berlin, 6Julius Wolff Institute (JWI) and Center for Musculoskeletal Surgery, Charité – Universitätsmedizin Berlin, Berlin, Germany, corporate member of Freie Universität Berlin,Humboldt-Universität zu Berlin
Chapters
- 00:04Title
- 00:58Adipose Tissue Digestion
- 03:37Liver Tissue Digestion
- 05:56Surface Staining and Flow Cytometric Analysis
- 07:53Results: Representative Leukocyte Population Analysis in a Nonalcoholic Steatohepatitis Mouse Model
- 09:46Conclusion
En enkel og pålitelig diett-indusert gnager dyr modell for alkoholfrie steatohepatitis (NASH) er beskrevet, oppnås gjennom ikke-SPF boliger av dyrene og administrasjon av en bestemt høy-fett diett. Vi beskriver identifisering av lever-og fett immunceller undergrupper for å recapitulate menneskelige immunologiske tilstander ved å utsette mus for miljø bakterier.
