A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Title
- 00:33Interscapular Brown Adipose Tissue (BAT) and Posterior White Adipose Tissue (WAT) Identification and Isolation
- 02:38Gonadal WAT Identification and Isolation
- 03:47Perirenal and Retroperitoneal WAT Identification and Isolation
- 04:33Other Adipose Depot Identification and Isolation
- 05:14Results: Representative Gross Anatomical Identification and Histological Evaluation of Mouse Adipose Depots
- 06:02Conclusion
Adipocytes exist in discrete depots and have diverse roles within their unique microenvironments. As regional differences in adipocyte character and function are uncovered, standardized identification and isolation of depots is crucial for advancement of the field. Herein, we present a detailed protocol for the excision of various mouse adipose depots.
