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Immunology and Infection

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JoVE Journal Immunology and Infection
Toxicity Study of Zinc Oxide Nanoparticles in Cell Culture and in Drosophila melanogaster
 

Toxicity Study of Zinc Oxide Nanoparticles in Cell Culture and in Drosophila melanogaster

Article DOI: 10.3791/59510-v • 06:47 min • September 19th, 2019
September 19th, 2019 •
1, 2, 3, 4, 4
1NUS Environmental Research Institute & Department of Anatomy, National University of Singapore, 2NUS Environmental Research Institute & School of Public Health, National University of Singapore, 3Department of Civil and Environmental Engineering, National University of Singapore, 4Department of Anatomy, National University of Singapore

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Summary
Automatic Translation

We describe a detailed protocol for evaluating the toxicological profiles of zinc oxide nanoparticles (ZnO NPs) in particular, the type of cell death in human MRC5 lung fibroblasts and ROS formation in the fruit fly Drosophila.

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Keywords: Nanotoxicity Cell Death Reactive Oxygen Species Fluorescence Activated Cell Sorting DHE Probe Zinc Oxide Nanoparticles MRC5 Human Lung Fibroblasts FITC Annexin V Propidium Iodide Flow Cytometry Drosophila Melanogaster Fly Food
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