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Droplet Digital TRAP (ddTRAP): Adaptation of the Telomere Repeat Amplification Protocol to Droplet Digital Polymerase Chain Reaction
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Cancer Research
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JoVE Journal Cancer Research
Droplet Digital TRAP (ddTRAP): Adaptation of the Telomere Repeat Amplification Protocol to Droplet Digital Polymerase Chain Reaction

Droplet Digital TRAP (ddTRAP): Adaptation of the Telomere Repeat Amplification Protocol to Droplet Digital Polymerase Chain Reaction

DOI:
10.3791/59550-v

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06:38 min

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May 03, 2019

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, ,
1School of Kinesiology,University of Michigan

Chapters

  • 00:04Title
  • 00:27Cell Lysis and Telomerase Extension Reaction
  • 01:43Droplet Digital PCR Setup
  • 03:21Detection of Telomerase Extension Products and Data Analysis
  • 05:03Results: Telomerase Activity
  • 05:51Conclusion

Summary

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We successfully converted the standard telomere repeat amplification protocol (TRAP) assay to be employed in droplet digital polymerase chain reactions. This new assay, called ddTRAP, is more sensitive and quantitative, allowing for better detection and statistical analysis of telomerase activity within various human cells.

Tags

Keywords: DdTRAPTelomere Repeat Amplification ProtocolDroplet Digital PCRTelomerase ActivityCell LysisExtension ReactionDdPCR Master MixDroplet Generation

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