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Isolering og rensning af murine hjerte Pericytes
JoVE Journal
Medicine
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JoVE Journal Medicine
Isolation and Purification of Murine Cardiac Pericytes
DOI:

10:49 min

August 16, 2019

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Chapters

  • 00:04Title
  • 00:44Preparation of Animal, Procurement of Cardiac Tissue, and Dissociation of Heart Tissue
  • 03:28Preparation of the Crude Cell Mixture for Fluorescence Activating Cell Sorting (FACS)
  • 06:34Purification of Pericytes from Crude Cell Mixture Using FACS
  • 08:18Results: Analysis of Crude Cells and Characterization of Primary Isolated Cardiac Pericytes
  • 09:55Conclusion

Summary

Automatic Translation

Vi har optimeret en protokol til at isolere og rense murine hjerte pericytes for grundforskning og undersøgelse af deres biologi og terapeutiske potentiale.

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