Journal
/
Biology
/
使用微蒂特萝卜无线电标签进行多维欧大肠杆菌测量 (p) ppGpp,然后是薄层色谱
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli (p)ppGpp Followed by Thin Layer Chromatography
Please note that all translations are automatically generated. Click here for the English version.

使用微蒂特萝卜无线电标签进行多维欧大肠杆菌测量 (p) ppGpp,然后是薄层色谱

DOI:
10.3791/59595-v

•

06:30 min

•

June 04, 2019

•

,
1Intramural Research Program,Eunice Kennedy Shriver NICHD, NIH

Chapters

  • 00:04Title
  • 00:47Labeling with 32P
  • 02:11Induction of Stress or Starvation and Sample and ppGpp Extraction
  • 02:54Thin-layer Chromatography and Quantitation of (p)ppGpp
  • 04:13Measurement of the Rate of ppGpp Decay
  • 04:46Results: In Vivo Measurements of Escherichia coli
  • 05:51Conclusion

Summary

Automatic Translation

Automatic Translation

微酸度皿中放射性标记细菌培养物的生长有助于高通量采样,从而允许对核苷酸池丰度进行多种技术和生物复制检测,包括 (p) ppGpp。可以监测生理压力来源引起的生长转变以及压力恢复的影响。

Tags

Microtiter DishRadiolabelingEscherichia Coli(p)ppGppThin Layer ChromatographyGlobal RegulatorBacterial CulturesStressMOPS MediaP32 OrthophosphateFormic AcidPEI Cellulose Thin Layer Chromatograms

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59595?article_type=v

MALDI样品制备：超薄层的方法
check_url/59595?article_type=v

用于测量的活性测定大肠埃希氏菌诱导赖氨酸Decarboxyase
check_url/59595?article_type=v

拟南芥极地甘油脂分析薄层色谱（TLC）再加上气相色谱（GLC）
check_url/59595?article_type=v

真菌内切葡聚糖酶的活性高通量筛选大肠埃希氏菌</em
check_url/59595?article_type=v

在体内肝细胞的分离纯化的肝细胞内吞其次是肝脏灌注
check_url/59595?article_type=v

测绘细菌功能的网络和途径<em>大肠杆菌</em使用合成基因阵列
check_url/59595?article_type=v

对于电感受的制备快速协议<em>大肠杆菌</em>和<em>霍乱弧菌</em
check_url/59595?article_type=v

检测现场的<em>大肠杆菌</em> O157：H7细胞的PMA-qPCR的
check_url/59595?article_type=v

在造血干细胞和祖细胞的成年小鼠颅骨骨髓体内四维跟踪
check_url/59595?article_type=v

蛋白质的共表达了多方面的好处<em>大肠杆菌</em

Read Article