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Funktionalisering af atomkraften mikroskop Cantihåndtag med enkelt-T-celler eller enkelt-partikel for immunologisk enkelt celle kraft spektroskopi
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Immunology and Infection
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JoVE Journal Immunology and Infection
Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Funktionalisering af atomkraften mikroskop Cantihåndtag med enkelt-T-celler eller enkelt-partikel for immunologisk enkelt celle kraft spektroskopi

DOI:
10.3791/59609-v

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10:06 min

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July 10, 2019

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, , ,
1State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine,Peking Union Medical College, 2Institute for Immunology, School of Medicine,Tsinghua University

Chapters

  • 00:04Title
  • 01:17AFM Cantilever Preparation
  • 02:36Attaching Single T-cells to the Cantilever
  • 04:58Force Spectroscopy of Single-pair T-cell/Dendritic Cell Interaction
  • 05:34Cantilever Functionalization with Single Polystyrene Beads
  • 07:44Results: Measuring Cell-Cell Adhesions
  • 09:30Conclusion

Summary

Automatic Translation

Automatic Translation

Vi præsenterer en protokol til funktionalisere atomkraften mikroskop (AFM) cantihåndtag med en enkelt T-celle og perle partikel for immunologiske undersøgelser. Der vises procedurer til sonde af enkeltpar T-celle-dendritisk cellebinding med AFM og overvågning af makro Fages realtids cellulære respons på en enkelt fast partikel ved AFM med fluorescens dannelse.

Tags

Atomic Force MicroscopeCantilever FunctionalizationSingle T CellSingle ParticleImmunological Force SpectroscopyDendritic Cell-T Cell InteractionCell-surface InteractionBiocompatible GlueIL-2Sample PreparationCalibrationLiving Cell Environment

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