Journal
/
Developmental Biology
/
Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

DOI:
10.3791/59634-v

•

09:56 min

•

April 30, 2019

•

, ,
1MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences,University of Edinburgh

Chapters

  • 00:04Title
  • 00:51Micropattern Manufacturing Procedure
  • 02:43Seeding Procedure
  • 03:44Fixation
  • 04:32Immunostaining
  • 05:19Imaging and Image Analysis
  • 07:55Results: Representative Micropattern Shape Analyses
  • 09:25Conclusion

Summary

Automatic Translation

Automatic Translation

The method presented here uses micropatterning together with quantitative imaging to reveal spatial organization within mammalian cultures. The technique is easy to establish in a standard cell biology laboratory and offers a tractable system to study patterning in vitro.

Tags

Keywords: MicropatternsQuantitative ImagingCell OrganizationIn Vitro ModelingCell PatterningCell DifferentiationCell ProliferationCell-to-cell CompetitionMicropatterning OptimizationCell AdhesionPhoto MaskUV Ozone IlluminationMatrix Deposition

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59634?article_type=v

Generation of Aggregates of Mouse Embryonic Stem Cells that Show Symmetry Breaking, Polarization and Emergent Collective Behaviour In Vitro
check_url/59634?article_type=v

Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System
check_url/59634?article_type=v

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates
check_url/59634?article_type=v

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
check_url/59634?article_type=v

Spatial and Temporal Analysis of Active ERK in the C. elegans Germline
check_url/59634?article_type=v

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps
check_url/59634?article_type=v

Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange
check_url/59634?article_type=v

Horizontal Whole Mount: A Novel Processing and Imaging Protocol for Thick, Three-dimensional Tissue Cross-sections of Skin
check_url/59634?article_type=v

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton
check_url/59634?article_type=v

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

Read Article