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Bioengineering

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JoVE Journal Bioengineering
In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin
 

In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin

Article DOI: 10.3791/59647-v • 05:45 min • July 11th, 2019
July 11th, 2019 •
1, 1, 1, 1
1School of Brain and Behavioral Science, Center for Advanced Pain Studies, University of Texas at Dallas

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Summary
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Morphological changes occur in immune responsive fibroblast cells following activation and promote alterations in cellular recruitment. Utilizing 2-photon imaging in conjunction with a genetically engineered Fibroblast-specific protein 1 (FSP1)-cre; tdTomato floxed-stop-floxed (TB/TB) mouse line and green fluorescently tagged lipopolysaccharide-FITC, we can illustrate highly specific uptake of lipopolysaccharide in dermal fibroblasts and morphological changes in vivo.

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Keywords: 2-photon Imaging Genetically-tagged Reporter Cells Skin In Vivo Fluorescent Proteins Mouse Paw Anesthesia Stereotaxic Apparatus Laser Excitation Focal Plane
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