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Journal / Bioengineering / A Multilayer Microfluidic Platform for the…
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Bioengineering

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JoVE Journal Bioengineering
A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
 

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Article DOI: 10.3791/59655-v • 11:23 min • October 6th, 2019
October 6th, 2019 •
1, 2, 1, 3, 2, 3, 1,2
1Institute for Complex Molecular Systems, Department of Biomedical Engineering, Computational Biology Group, Eindhoven University of Technology, 2Institute for Molecules and Materials, Radboud University, 3Institute of Bioengineering, School of Engineering École Polytechnique Fédérale de Lausanne (EPFL)

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Summary
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The fabrication process of a PDMS-based, multilayer, microfluidic device that allows in vitro transcription and translation (IVTT) reactions to be performed over prolonged periods is described. Furthermore, a comprehensive overview of the hardware and software required to automate and maintain these reactions for prolonged durations is provided.

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Keywords: Microfluidic Platform Cell-free Gene Expression Synthetic Gene Networks Prototyping GFP Biochemical Reactions Flow Reactor Pneumatic Control Peltier Cooling PTFE Tubing PEEK Tubing Temperature Control
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