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A Human Peripheral Blood Mononuclear Cell (PBMC) Engrafted Humanized Xenograft Model for Translational Immuno-oncology (I-O) Research
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Cancer Research
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JoVE Journal Cancer Research
A Human Peripheral Blood Mononuclear Cell (PBMC) Engrafted Humanized Xenograft Model for Translational Immuno-oncology (I-O) Research

A Human Peripheral Blood Mononuclear Cell (PBMC) Engrafted Humanized Xenograft Model for Translational Immuno-oncology (I-O) Research

DOI:
10.3791/59679-v

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08:17 min

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August 15, 2019

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, , , , , , , , , , , , , ,
1BeiGene (Beijing) Co., Ltd.

Chapters

  • 00:04Title
  • 00:46Cyclophosphamide Myeloablation and Human Peripheral Blood Mononuclear Cell (PBMC) Transplantation and Tumor Engraftment
  • 02:08Immunohistochemistry (IHC)
  • 04:33In Vivo Efficacy and Pharmacodynamics Analyses
  • 06:08Results: Representative PBMC- and Tumor-Engraftment and Anti-Tumor Antibody Treatment Analysis
  • 07:16Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a human peripheral blood mononuclear cell (PBMC) — based humanized xenograft mouse model for translational immuno-oncology research. This protocol could serve as a general guideline for establishing and characterizing similar models for I-O therapy assessment.

Tags

PBMCHumanized XenograftImmuno-oncologyMyeloid Cell DepletionCyclophosphamideDisulfiramTumor GrowthImmunohistochemistryAntigen Retrieval

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