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Generation of Tumor Organoids from Genetically Engineered Mouse Models of Prostate Cancer
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Genetics
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JoVE Journal Genetics
Generation of Tumor Organoids from Genetically Engineered Mouse Models of Prostate Cancer

Generation of Tumor Organoids from Genetically Engineered Mouse Models of Prostate Cancer

DOI:
10.3791/59710-v

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08:54 min

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June 13, 2019

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, , ,
1Department of Pharmacology & Therapeutics,Roswell Park Comprehensive Cancer Center

Chapters

  • 00:04Title
  • 00:30En Bloc Male Urogenital System Extraction
  • 01:08Pelvic Lymph Node, Spleen, Liver, Kidney, Lung, Tibia, and Femur Extraction
  • 03:16Prostate Tumor Dissection
  • 05:17Tumor Tissue Digestion
  • 06:07Matrix Dome Plating
  • 07:07Results: Representative Solid and Fluid-filled Tumor Characterization
  • 08:28Conclusion

Summary

Automatic Translation

Automatic Translation

We show a method for necropsy and dissection of mouse prostate cancer models, focusing on prostate tumor dissection. A step-by-step protocol for generation of mouse prostate tumor organoids is also presented.

Tags

Keywords: Tumor OrganoidsGenetically Engineered Mouse ModelsProstate Cancer3D Cell CultureIn Vitro Cancer ResearchMouse Urogenital SystemMetastatic LesionsLymph NodesOrgan Dissection

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