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评估癌细胞3D球形培养中的细胞活力和死亡
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells
DOI:

10:33 min

June 16, 2019

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Chapters

  • 00:04Title
  • 00:59Spontaneous Spheroid Formation
  • 01:45Reconstituted Basement Membrane-mediated Spheroid Formation
  • 03:04Hanging Drop Spheroids
  • 04:14Preparing Protein Lysates for Western Blotting from 3D Spheroid Cultures
  • 05:21Propidium Iodide (PI) Staining of Spheroids
  • 06:27Embedding of Spheroids
  • 07:52Results: Assessing Cell Viability and Death
  • 09:24Conclusion

Summary

Automatic Translation

在这里,我们提出了几种简单的方法来评估3D癌细胞球体的生存能力和死亡,它模仿体内肿瘤的物理化学梯度比2D培养要好得多。因此,球形模型允许评估癌症药物疗效,并改进对体内条件的翻译。

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