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Immunology and Infection

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链球菌中高分子质量蛋白的纯化

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Immunology and Infection

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JoVE Journal Immunology and Infection

Purification of a High Molecular Mass Protein in Streptococcus mutans

Please note that all translations are automatically generated. Click here for the English version.

链球菌中高分子质量蛋白的纯化

DOI:

10.3791/59804-v

•

09:51 min

•

September 14, 2019

•

Takatoshi Murata, Mamiko Yamashita, Masao Ishikawa², Koji Shibuya, Nobuhiro Hanada

¹Department of Translational Research,Tsurumi University School of Dental Medicine, ²Laboratory for Oral Health Science

Chapters

00:06Title
00:51PCR Amplification
02:38Cell Transformation
03:35Purification of Polyhistidine-tagged GTF-SI
07:29Results: Generation of S. mutans His-gtfC and Functional Restoration
08:59Conclusion

Summary

Automatic Translation

这里描述的是一个简单的方法，用于纯化链球菌中的基因产物。该技术在蛋白质，特别是膜蛋白和高分子质量蛋白的纯化方面可能有利，可用于其他各种细菌物种。

Tags

Protein Purification Streptococcus Mutans High Molecular Mass Protein PCR Genomic DNA Extraction Gel Electrophoresis Gel Extraction Nested PCR Electroporation Competent Cells Brain-heart Infusion Broth

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