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Genetics
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人体血液的突变载荷和克隆成分的特征
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal
Genetics
Characterizing Mutational Load and Clonal Composition of Human Blood
Please note that all translations are automatically generated.
Click here for the English version.
人体血液的突变载荷和克隆成分的特征
DOI:
10.3791/59846-v
•
07:58 min
•
July 11, 2019
•
Axel Rosendahl Huber
,
Freek Manders
,
Rurika Oka
,
Ruben van Boxtel
1
Princess Máxima Center for Pediatric Oncology
Chapters
00:04
Title
00:41
HSPC Isolation, Sorting, and Culture
02:29
Harvesting HSPC Clones
04:05
Construction of a Developmental Lineage Tree Using Base Substitutions
06:05
Results: Checking Quality After Sequencing and Mutation Analysis, and Constructing a Developmental Lineage Tree
07:38
Conclusion
Summary
Automatic Translation
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Automatic Translation
细胞体突变模式反映先前突变暴露,可以揭示发育系系关系。这里介绍的是一种对单个造血干细胞和祖细胞的体细胞突变进行编目和分析的方法。
Tags
Mutational Load
Clonal Composition
Hematopoietic Stem Cell
DNA Repair
Mutagenic
Cell Sorting
HSPC
CD34
CD38
CD45RA
Clone
Cell Culture
Single Cell
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