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Intravital Imaging of Intraepithelial Lymphocytes in Murine Small Intestine
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Immunology and Infection
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JoVE Journal Immunology and Infection
Intravital Imaging of Intraepithelial Lymphocytes in Murine Small Intestine

Intravital Imaging of Intraepithelial Lymphocytes in Murine Small Intestine

DOI:
10.3791/59853-v

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08:00 min

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June 24, 2019

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1Center for Immunity and Inflammation, Department of Pathology, Immunology and Laboratory Medicine,Rutgers New Jersey Medical School

Chapters

  • 00:04Title
  • 00:58Intestinal Mucosa Exposure
  • 03:41Spinning Disk Confocal Microscopy Imaging
  • 05:59Results: Representative Gamma-delta Intraepithelial Lymphocyte (IEL) Behavior Analyses
  • 07:15Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a method to visualize GFP-labeled γδ IELs using intravital imaging of murine small intestine by inverted spinning disk confocal microscopy. This technique enables the tracking of live cells within the mucosa for up to 4 h and can be used to investigate a variety of intestinal immune-epithelial interactions.  

Tags

Keywords: Intravital ImagingIntraepithelial LymphocytesMurine Small IntestineMicroscopyImmune Cell InteractionsEpitheliumReal-time ImagingMucosal ImmunityEpithelial BiologyCancer BiologySurgical TechniqueAnesthetized Transgenic MouseEGFPGamma-delta T-cell ReceptorHoechst 33342 DyeAbdominal IncisionMesenteryPeritoneal CavitySuture Placement

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