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Immunology and Infection
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调整降解以实现特定和高效的蛋白质消耗
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Tuning Degradation to Achieve Specific and Efficient Protein Depletion
Please note that all translations are automatically generated.
Click here for the English version.
调整降解以实现特定和高效的蛋白质消耗
DOI:
10.3791/59874-v
•
05:11 min
•
July 20, 2019
•
J. David Barrass
,
Gonzalo I. Mendoza-Ochoa
2
,
Isabella E. Maudlin
3
,
Emanuela Sani
,
Jean D. Beggs
1
Wellcome Centre for Cell Biology, School of Biological Sciences
,
University of Edinburgh
,
2
Department of Plant Sciences
,
University of Cambridge
,
3
Sir William Dunn School of Pathology
,
University of Oxford
Chapters
00:04
Title
00:36
General Procedure for Depletion
03:31
Results: Analysis of the Tuned Depletion Rate
04:25
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出一个协议,使用β-est AID系统,有效和具体地耗尽酵母
糖
精中感兴趣的蛋白质。
Tags
Protein Depletion
Essential Protein
Protein Function
Protein Removal
Auxin
Beta-estradiol
IAA
Cell Death
Cell Culture
Optical Density
Sample Collection
Centrifugation
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