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制备和利用新鲜分离的人类分离器平滑肌细胞,用于9-苯酚敏感阳离子电流的表征
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents
DOI:

08:30 min

January 31, 2020

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Chapters

  • 00:04Introduction
  • 01:45Dissection and Enzymatic Dissociation of Mucosa-free DSM Pieces
  • 03:28Voltage-step Induced Cation Currents from DSM Cells Using Amphotericin-B Perforated Whole-cell Voltage Patch-clamp Technique
  • 05:52Results: Investigation of Ion Channel Properties in Isolated DSM Cells
  • 07:07Conclusion

Summary

Automatic Translation

我们描述了一种从人类膀胱标本中制备单一新分离的分离性肌细胞的方法,采用两步酶程序。获得可行的DSM细胞可以通过各种单细胞技术进行研究,包括所述的amphotericin-B贴片-钳电生理学,以揭示生理和药理特性。

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