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In Vitro Assay to Study Tumor-macrophage Interaction
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JoVE Journal Cancer Research
In Vitro Assay to Study Tumor-macrophage Interaction

In Vitro Assay to Study Tumor-macrophage Interaction

DOI:
10.3791/59907-v

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08:36 min

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August 01, 2019

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,
1Department of Neurology,University of California, San Francisco, 2Helen Diller Family Comprehensive Cancer Center,University of California, San Francisco, 3Departments of Pediatrics and Neurological Surgery,University of California, San Francisco

Chapters

  • 00:04Title
  • 00:40Splitting Tumor Cells
  • 03:14In Vitro Macrophage Attraction Assay: Preparation of the Conditioned Media
  • 04:55In Vitro Macrophage Attraction Assay: Preparation of MV-4-11 Cells
  • 06:03In Vitro Macrophage Attraction Assay: Transwell Assay
  • 07:25Results: Analysis of the Assay and its Ability to Study Tumor-macrophage Interaction
  • 08:02Conclusion

Summary

Automatic Translation

Automatic Translation

This article represents a useful in vitro assay to evaluate the capability of conditioned medium from tumor cells to attract macrophages.

Tags

Keywords: Tumor-associated MacrophagesTumor MicroenvironmentGenetic MutationsPersonalized TreatmentIn Vitro AssayTumor-macrophage InteractionImmune CellsCell DetachmentCell CountingCell Seeding

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