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Påvisning av 5-Hydroxymethylcytosine i nevrale stamceller og hjerner av mus
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Neuroscience
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JoVE Journal Neuroscience
The Detection of 5-Hydroxymethylcytosine in Neural Stem Cells and Brains of Mice

Påvisning av 5-Hydroxymethylcytosine i nevrale stamceller og hjerner av mus

DOI:
10.3791/59950-v

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08:03 min

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September 19, 2019

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, , , ,
1The Children’s Hospital, School of Medicine,Zhejiang University, 2The Institute of Translational Medicine, School of Medicine,Zhejiang University

Chapters

  • 00:04Title
  • 01:00Transcardial Perfusion of the Mouse
  • 02:03Brain Sectioning and Immunofluorescence Staining
  • 04:02Genomic DNA Isolation
  • 05:43DNA Dot Blot
  • 07:03Results: Immunofluorescence Staining and Dot Blot Detection
  • 07:49Conclusion

Summary

Automatic Translation

Automatic Translation

Her presenterer vi en protokoll for å oppdage 5-hydroxymethylcytosine i celler og hjernen vev, utnytte immunofluorescence farging og DNA dot-blot metoder.

Tags

5-hydroxymethylcytosine5hmCImmunofluorescenceDNA Dot-blotNeural Stem CellsMouse BrainEpigenetic ModificationGene ExpressionNeurological DisordersPerfusionFixationCryosectioningImmunostaining

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