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JoVE Journal Genetics
Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage
 

Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage

Article DOI: 10.3791/59955-v • 11:30 min • March 24th, 2020
March 24th, 2020 •
*1, 1, 1, 2
1Toxicology Program, ILS, Inc., 2Division of the National Toxicology Program, National Institute of Environmental Health Sciences
* These authors contributed equally

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Summary
Automatic Translation

This protocol describes several procedures for preparing high quality frozen tissue samples at the time of necropsy for use in the comet assay to assess DNA damage: 1) minced tissue, 2) scraped epithelial cells from the gastrointestinal tract, and 3) cubed tissue samples, requiring homogenization using a tissue mincing device.

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Keywords: Comet Assay Frozen Tissue DNA Damage Genotoxicity Pre-clinical Drug Development Safety Assessment Environmental Biomonitoring Tissue Processing Sample Handling
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