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Cell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry
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Cancer Research
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JoVE Journal Cancer Research
Cell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry

Cell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry

DOI:
10.3791/59968-v

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08:21 min

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September 01, 2019

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, , , , ,
1CCU Molecular and Radiation Oncology,German Cancer Research Center, 2Department of Radiation Oncology,Heidelberg University Hospital, 3Department of Radiation Oncology,Freiburg University Medical Center

Chapters

  • 00:04Title
  • 00:56Sample Collection
  • 01:44Cell Staining
  • 02:45Analysis
  • 04:28Data Evaluation
  • 06:15Results: DNA Damage Response After Different Types of Radiation
  • 07:24Conclusion

Summary

Automatic Translation

Automatic Translation

The presented method combines the quantitative analysis of DNA double-strand breaks (DSBs), cell cycle distribution and apoptosis to enable cell cycle-specific evaluation of DSB induction and repair as well as the consequences of repair failure.

Tags

Keywords: Cell CycleDNA DamageGenotoxic StressFlow CytometryγH2AXApoptosisRadiobiologyRadiotherapyOncologyDNA StainingCell FixationCell Suspension

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