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1Laboratory of Molecular Biology, Department of Plant Sciences,Wageningen University & Research, 2Center of Technology for Agricultural Production,Agency for the Assessment and Application of Technology (BPPT), 3Department of Ecological Science, Faculty of Earth and Life Sciences,Vrije Universiteit Amsterdam, 4Beijing Advanced Innovation Center for Tree Breeding by Molecular Design,Beijing University of Agriculture
Chapters
- 00:04Title
- 01:08Growth of P. andersonii Trees
- 03:14Stable transformation of P. andersonii
- 06:40Preparation of Rooted P. andersonii Plantlets
- 07:31Nodulation of P. andersonii Plantlets in Pots
- 09:48Results: Stable Transformation Procedure
- 11:13Conclusion
Parasponia andersonii is a fast-growing tropical tree that belongs to the Cannabis family (Cannabaceae) and can form nitrogen-fixing root nodules in association with the rhizobium. Here, we describe a detailed protocol for reverse genetic analyses in P. andersonii based on Agrobacterium tumefaciens-mediated stable transformation and CRISPR/Cas9-based genome editing.
