A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Division of Nephrology and Clinical Immunology,University Hospital RWTH Aachen, 2III. Department of Medicine,University Medical Center, Hamburg-Eppendorf, 3Department of Nephrology,Monash Health, 4Division of Nephrology and Hypertension,Oregon Health and Science University, 5Renal Section,Veterans Affairs Portland Health Care System, 6Fondation LeDucq Transatlantic Networks of Excellence, 7Department of Internal Medicine, Nephrology and Transplantation,Erasmus Medical Center
Chapters
- 00:04Title
- 01:11Fixation and Immunostaining of Mouse Kidneys
- 03:26Tissue Clearing and Imaging
- 04:13Results: Immunolabeled and Optically Cleared Kidney Tissue
- 05:23Conclusion
La combinación de etiquetado de anticuerpos, limpieza óptica y microscopía de luz avanzada permite el análisis tridimensional de estructuras u órganos completos. Aquí se describe un método sencillo para combinar la inmunoetiquetado de rodajas renales gruesas, la limpieza óptica con la canela etílico y la imagen confocal que permite la visualización y cuantificación de estructuras renales tridimensionales.
