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Inducing meningokokk hjernehinnebetennelse serogruppe C i mus via Intracisternal Delivery
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal Delivery
DOI:

10:03 min

November 05, 2019

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Chapters

  • 00:05Title
  • 01:48Preparation of Bacteria for In Vivo Infection Studies
  • 03:09Intra-cisternal Injection of Mice
  • 05:07Evaluation of Colony Forming Units (CFU) Counts in Peripheral Organs
  • 06:39Preparation of Brain Tissues for CFU Count
  • 08:05Results: Survival of Mice Following Intracisternal Injection of Wild-type and Mutant N. meningitidis
  • 09:28Conclusion

Summary

Automatic Translation

Her beskriver vi en metode for å indusere meningokokk meningitt gjennom en intracisternal ruten av infeksjon i voksen mus. Vi presenterer en trinnvis protokoll for meningokokkinfeksjon fra utarbeidelse av inokulum til intracisternal infeksjon; deretter registrere dyret overlevelse og evaluere bakteriell belastninger i murine vev.

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