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Immunology and Infection
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人类S100A12及其用于免疫细胞刺激的Ion诱导寡聚物的纯化
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation
Please note that all translations are automatically generated.
Click here for the English version.
人类S100A12及其用于免疫细胞刺激的Ion诱导寡聚物的纯化
DOI:
10.3791/60065-v
•
12:55 min
•
September 29, 2019
•
Sabrina Fuehner
,
Dirk Foell
,
Christoph Kessel
1
Department of Pediatric Rheumatology and Immunology
,
University Children’s Hospital
Chapters
00:05
Title
01:02
Anion-exchange Chromatography
03:53
Calcium-dependent Hydrophobic-interaction Chromatography (HIC)
05:26
Removal of Endotoxin and Oligomer Separation
07:20
Functional Testing on Monocytes
10:22
Results: Oligomers Separation
11:59
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议描述了一种用于重组无标记标记的钙结合蛋白S100A12及其用于人类单核细胞刺激测定的由水因诱导的寡聚物的纯化方法。
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