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Rensing av Human S100A12 og Its ion-indusert Oligomers for immun celle stimulering
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Immunology and Infection
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JoVE Journal Immunology and Infection
Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation

Rensing av Human S100A12 og Its ion-indusert Oligomers for immun celle stimulering

DOI:
10.3791/60065-v

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12:55 min

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September 29, 2019

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, ,
1Department of Pediatric Rheumatology and Immunology,University Children’s Hospital

Chapters

  • 00:05Title
  • 01:02Anion-exchange Chromatography
  • 03:53Calcium-dependent Hydrophobic-interaction Chromatography (HIC)
  • 05:26Removal of Endotoxin and Oligomer Separation
  • 07:20Functional Testing on Monocytes
  • 10:22Results: Oligomers Separation
  • 11:59Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokollen beskriver en rensing metode for rekombinant tag-Free kalsium bindende protein S100A12 og Ion-indusert oligomers for menneskelig monocytt stimulering analyser.

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