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A High-Throughput In Situ Method for Estimation of Hepatocyte Nuclear Ploidy in Mice
JoVE Journal
Medicine
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JoVE Journal Medicine
A High-Throughput In Situ Method for Estimation of Hepatocyte Nuclear Ploidy in Mice
DOI:

08:44 min

April 19, 2020

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Chapters

  • 00:05Introduction
  • 01:14Cyrosectioning
  • 01:54Fluorescence Immunolabelling
  • 03:55Fluorescence Image Acquisition and Automated Fluorescence Image Analysis
  • 04:55Data Analysis
  • 06:24Results: Representative Murine Hepatocyte Nuclear Ploidy Estimation
  • 08:03Conclusion

Summary

Automatic Translation

We present a robust, cost-effective, and flexible method for measuring changes in hepatocyte number and nuclear ploidy within fixed/cryopreserved tissue samples that does not require flow cytometry. Our approach provides a powerful sample-wide signature of liver cytology ideal for tracking the progression of liver injury and disease.

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