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Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography
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Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography

Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography

DOI:
10.3791/60104-v

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11:01 min

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January 18, 2020

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, , , , , , , , ,
1Beijing Key Laboratory of Clinical Epidemiology, School of Public Health,Capital Medical University, 2School of Public Health,Shandong First Medical University & Shandong Academy of Medical Sciences, 3School of Medical and Health Sciences,Edith Cowan University

Chapters

  • 00:05Title
  • 01:42Immunoglobulin G (IgG) Isolation
  • 03:57Glycan Release
  • 05:25Glycan Labeling and Purification
  • 07:30Hydrophilic Interaction Chromatography and Analysis of Glycans
  • 08:52Results: UPLC Chromatogram and Structure
  • 09:30Conclusion

Summary

Automatic Translation

Automatic Translation

Immunoglobulin G (IgG) N-glycan is characterized using hydrophilic interaction chromatography UPLC. In addition, the structure of IgG N-glycan is clearly separated. Presented here is an introduction to this experimental method so that it can be widely used in research settings.

Tags

Keywords: Immunoglobulin GN-glycan AnalysisUltra-performance Liquid ChromatographyIgG PurificationProtein G Monolithic PlateSample PreparationGlycan IsomersPlasma SamplesGlycan Analysis

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