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Biaxial Basal Tone and Passive Testing of the Murine Reproductive System Using a Pressure Myograph
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Bioengineering
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JoVE Journal Bioengineering
Biaxial Basal Tone and Passive Testing of the Murine Reproductive System Using a Pressure Myograph

Biaxial Basal Tone and Passive Testing of the Murine Reproductive System Using a Pressure Myograph

DOI:
10.3791/60125-v

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09:59 min

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August 13, 2019

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, , , , ,
1Department of Biomedical Engineering,Tulane University

Chapters

  • 00:04Title
  • 00:59Reproductive System Dissection and Cannulation
  • 02:15Basal Tone Mechanical Testing
  • 05:55Passive Mechanical Testing
  • 07:54Results: Representative Biaxial Basal Tone and Passive Murine Reproductive System Testing
  • 09:06Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol utilized a commercially available pressure myograph system to perform pressure myograph testing on the murine vagina and cervix. Utilizing media with and without calcium, the contributions of the smooth muscle cells (SMC) basal tone and passive extracellular matrix (ECM) were isolated for the organs under estimated physiological conditions.

Tags

Biaxial TestingPressure MyographReproductive SystemCervixVaginaSmooth MusclePassive MatrixMechanical PropertiesWomen’s HealthBiomechanics

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