A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Biomedical and Molecular Sciences and Department of Pathology and Molecular Medicine,Queen’s University, Kingston, 2Department of Chemical and Physical Sciences,University of Toronto, Mississauga, 3Department of Laboratory Medicine, Brampton Civic Hospital Campus,William Osler Health System, 4Latner Thoracic Surgery Research Laboratories
Chapters
- 00:05Introduction
- 01:24Plating and Differentiation Induction of HC11 Cells
- 03:363D Growth and Differentiation Induction of EpH4 Cells in EHS Matrix
- 06:24Western Blotting for β-Casein
- 07:42Results: Assessment of HC11 and EpH4 Cell Differentiation
- 08:49Conclusion
We describe techniques for differentiation induction of two breast epithelial lines, HC11 and EpH4. While both require fetal calf serum, insulin, and prolactin to produce milk proteins, EpH4 cells can fully differentiate into mammospheres in three-dimensional culture. These complementary models are useful for signal transduction studies of differentiation and neoplasia.
