JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Medicine
/
人类肠道器官脂滴形成特征化和定量的荧光测定
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Medicine
A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids
Please note that all translations are automatically generated.
Click here for the English version.
人类肠道器官脂滴形成特征化和定量的荧光测定
DOI:
10.3791/60150-v
•
10:12 min
•
October 13, 2019
•
Jorik M. van Rijn
2,3
,
Marliek van Hoesel
2
,
Sabine Middendorp
2
1
Division of Pediatrics, Department of Pediatric Gastroenterology, Wilhelmina Children’s Hospital
,
University Medical Center Utrecht, Utrecht University
,
2
Regenerative Medicine Center
,
University Medical Center Utrecht, Utrecht University
,
3
Science for Life Laboratory, Department of Medical Biochemistry and Microbiology
,
Uppsala University
Chapters
00:04
Title
01:15
Culture Procedures for Human Small Intestinal Organoids
04:05
Lipid Droplet Formation Assay
07:26
Results: Analysis of Lipid Drop Formation
09:29
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该协议描述了在脂肪酸刺激下人体肠道器官中脂液滴(LD)形成的表征测定。我们讨论了这种测定如何用于LD形成定量,以及如何用于影响LD形成的药物的高通量筛选。
Tags
Lipid Droplet
Intestinal Organoids
Fluorescence Assay
DGAT1 Deficiency
High-throughput Screening
Lipid Metabolism
Cell Culture
Trypsinization
Cell Counting
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
在
犬
体内的
肌肉功能测定
从人乳腺癌组织及细胞系微球体形成分析
软琼脂集落形成实验利用识别的致瘤性乳腺癌细胞抑制剂
建立和UTI和CAUTI表征在小鼠模型
后皮肤微移植生成一个新的方法解聚表征的组织
战略内皮细胞形成实验:比较外基质和生长因子,细胞外基质
毛喉素诱导在肠组织体溶胀:一个<em>体外</em>检测方法的评估囊性纤维化患者对药物的反应
荧光介导的断层扫描对巨噬细胞相关小鼠肠道炎症的检测和定量
一种基于动力学荧光的 Ca
2 +
动员法, 用于识别 G 蛋白偶联受体激动剂、拮抗器和构调制器
大鼠同时心电图记录及侵入性血压测量
Read Article