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人类肠道器官脂滴形成特征化和定量的荧光测定
JoVE Journal
Medicine
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JoVE Journal Medicine
A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids
DOI:

10:12 min

October 13, 2019

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Chapters

  • 00:04Title
  • 01:15Culture Procedures for Human Small Intestinal Organoids
  • 04:05Lipid Droplet Formation Assay
  • 07:26Results: Analysis of Lipid Drop Formation
  • 09:29Conclusion

Summary

Automatic Translation

该协议描述了在脂肪酸刺激下人体肠道器官中脂液滴(LD)形成的表征测定。我们讨论了这种测定如何用于LD形成定量,以及如何用于影响LD形成的药物的高通量筛选。

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