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A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids
 
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A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids

Article DOI: 10.3791/60150-v • 10:12 min • October 13th, 2019
October 13th, 2019 •
1,2,3, 1,2, 1,2
1Division of Pediatrics, Department of Pediatric Gastroenterology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht University, 2Regenerative Medicine Center, University Medical Center Utrecht, Utrecht University, 3Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, Uppsala University

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This protocol describes an assay for the characterization of lipid droplet (LD) formation in human intestinal organoids upon stimulation with fatty acids. We discuss how this assay is used for quantification of LD formation, and how it can be used for high throughput screening for drugs that affect LD formation.

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Keywords: Lipid Droplet Intestinal Organoids Fluorescence Assay DGAT1 Deficiency High-throughput Screening Lipid Metabolism Cell Culture Trypsinization Cell Counting
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