Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / In Situ Monitoring of Transiently Formed…
JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology
In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells
 

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells

Article DOI: 10.3791/60172-v • 08:58 min • September 2nd, 2019
September 2nd, 2019 •
*1, *2, 3
1Department of Biomedical Sciences of Cells & Systems, University of Groningen, 2Department of Anatomy, Faculty of Medicine, University of Colombo, 3Australian Regenerative Medicine Institute (ARMI), Monash University
* These authors contributed equally

Chapters

Summary
Automatic Translation

Cognate J-domain proteins cooperate with the Hsp70 chaperone to assist in a myriad of biological processes ranging from protein folding to degradation. Here, we describe an in situ proximity ligation assay, which allows the monitoring of these transiently formed chaperone machineries in bacterial, yeast and human cells.

Tags

Keywords: In Situ Monitoring Transient Protein Interactions Chaperone Complexes Proteostasis Prokaryotic And Eukaryotic Cells Microbial Infections Protein Assemblies Immunohistochemistry Immunofluorescence ELISA Immunoprecipitations Poly-L-Lysine Paraformaldehyde Triton-X100 S. Cerevisiae
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter