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分离的月毛心室肌细胞的光学成像
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Optical Imaging of Isolated Murine Ventricular Myocytes
DOI:

11:32 min

January 17, 2020

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Chapters

  • 00:05Title
  • 01:26Preparation of Coverslips
  • 02:08Myocyte Isolation
  • 05:53Membrane Potential Dye Loading
  • 07:01Photometry and Charge Coupled Device Recordings
  • 09:21Results: Calcium and Sarcomere Shortening
  • 10:48Conclusion

Summary

Automatic Translation

我们介绍了分离鼠肌细胞的方法,以及如何使用荧光光度测量与同时进行数字细胞几何测量,同时获得电压或钙痕迹。

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