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Bioengineering
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単離マウス心室筋細胞の光学イメージング
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Optical Imaging of Isolated Murine Ventricular Myocytes
Please note that all translations are automatically generated.
Click here for the English version.
単離マウス心室筋細胞の光学イメージング
DOI:
10.3791/60196-v
•
11:32 min
•
January 17, 2020
•
Shuxin Han
2
,
Matt Klos
,
Sherry Morgenstern
,
Ramiz Ahmad
,
Isabella Pua
,
Shreyas Suresh
,
Kayla Hicks
,
Eric Devaney
1
Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine
,
University of Science and Technology of China, Hefei, Anhui, China
,
2
Central Nodal (Anhui) Bioscience and Technology Research Center, Hefei, Anhui, China
,
3
Pediatric Cardiac and Thoracic Surgery
,
University Hospitals Cleveland Medical Center
Chapters
00:05
Title
01:26
Preparation of Coverslips
02:08
Myocyte Isolation
05:53
Membrane Potential Dye Loading
07:01
Photometry and Charge Coupled Device Recordings
09:21
Results: Calcium and Sarcomere Shortening
10:48
Conclusion
Summary
Automatic Translation
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Automatic Translation
マウス筋細胞の単離の方法論と、同時デジタル細胞幾何学的測定と蛍光光光測定を用いたサルコメア短縮トレースと同時に電圧またはカルシウムの痕跡を得る方法を提示する。
Tags
Optical Imaging
Murine Ventricular Myocytes
Heart Failure
Cardiac Metabolism
Excitation-contraction Coupling
Cardiac Myocyte Isolation
Fluorescent Probe
Cardiac Toxicity
Heart Failure Therapeutics
Transgenic Mice
Laminin
Langendorf Perfusion
KHB-HB Solution
Digestion Buffer
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